期刊
ENVIRONMENTAL TECHNOLOGY
卷 32, 期 11, 页码 1287-1294出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330.2010.535177
关键词
lignin peroxidase; veratryl alcohol; Gleophyllum striatum; n-propanol, polycyclic aromatic hydrocarbons; metalloenzyme
资金
- DST, Government of India, New Delhi [SR/WOS-A/CS-27/2004]
Lignin peroxidase has been purified to homogeneity using a process of concentration by ultrafiltration and anion exchange chromatography on diethylaminoethyl (DEAE) cellulose from the liquid culture filtrate of the brown rot fungi Gleophyllum striatum MTCC-1117. The molecular mass of the purified enzyme is 43 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The K-m values for the enzyme using veratryl alcohol, hydrogen peroxide and n-propanol were 66 mu M, 82 mu M and 476 mu M, respectively. The pH and temperature optima of the enzyme were 2.8 and 25 degrees C, respectively. The enzyme is completely inhibited by 20% of the water miscible organic solvents acetone dioxane, diethylether, acetonitrile and dimethylformamide. The lignin peroxidase oxidizes polycyclic aromatic hydrocarbons pyrene, acenaphthene, anthracene, dibenothiophene and 9-methyl anthracene.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据