期刊
ENVIRONMENTAL TECHNOLOGY
卷 29, 期 12, 页码 1357-1364出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330802379615
关键词
Response surface; laccase mediator system; optimisation; textile dye
资金
- LSRE [FEDER/POCI/2010]
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BD/28529/2006, SFRH/BPD/22697/2005]
A three-level Box-Behnken factorial design with three factors and the Response Surface Methodology were used to optimise the colour removal of the reactive textile dye, Colour Index (C.I.) reactive red 180, by commercial laccase. A mathematical model was developed to study the effect of temperature, pH, enzyme concentration and their interactions on the decolourisation. Enzyme concentration and pH as well as their interaction were the principal factors that affected the decolourisation. The dye degradation was independent of temperature. The model estimated that the highest decolourisation ( 92%) was obtained for 27C, pH 7.5 and 85 U l-1. This predicted value was experimentally validated, obtaining dye colour removal (540 nm) of 93 1.5%.
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