期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 21, 期 15, 页码 9385-9395出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-014-2872-1
关键词
Hydrocarbon degradation; Biosurfactant production; Pseudomonas sp P-1; Crude oil; Rhamnolipid; rhl gene
资金
- Polish Ministry of Science and Higher Education [N N523 418237]
- European Community from the European Social Fund
The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13 % of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. H-1 and C-13 nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.
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