期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 21, 期 18, 页码 10736-10743出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-014-3057-7
关键词
Vicia faba L; Root tip cells; Aluminum(Al); Nucleolus; Nucleophosmin; Fibrillarin; Nucleolin
资金
- National Natural Science Foundation of China [30972331]
- Scientific Research Foundation of Graduate School of South China Normal University [C1045210]
Results from our previous investigation indicated that Al could affect the nucleolus and induce extrusion of silver-staining nucleolar particles containing argyrophilic proteins from the nucleolus into the cytoplasm in root tip cells of Vicia faba L. So far, the nucleolar proteins involved have not been identified. It is well known that nucleophosmin (B23), nucleolin (C23), and fibrillarin are three major and multifunctional nucleolar proteins. Therefore, effects of Al on B23, C23, and fibrillarin in root tip cells of V. faba exposed to 100 mu M Al for 48 h were observed and analyzed using indirect immunofluorescence microscopy and Western blotting. The results from this work demonstrated that after 100 mu M of Al treatment for 48 h, B23 and C23 migrated from the nucleolus to the cytoplasm and fibrillarin from the nucleolus to the nucleoplasm. In some cells, fibrillarin was present only in the cytoplasm. Western blotting data revealed higher expression of the three major nucleolar proteins in Al-treated roots compared with the control and that the B23 content increased markedly. These findings confirmed our previous observations.
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