期刊
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 108, 期 -, 页码 72-82出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jri.2015.02.005
关键词
Chemokine receptor; Feto-maternal tolerance; Regulatory T cells; Paternal antigen; Seminal fluid
资金
- KAKENHI [23791818, 26861316, 24791107, 26670717, 23390386]
- Grants-in-Aid for Scientific Research [26861316, 26670717, 24791107, 23791818] Funding Source: KAKEN
Paternal antigen-specific regulatory T (PA-specific Treg) cells play an important role in feto-maternal tolerance. To detect the PA-specific Tregs, female BALB/c mice were mated with male DBA/2 mice. Mls Ia antigen on DBA/2 mice is recognized by the T-cell receptor V beta 6; thus, CD4(+)Foxp3(+)V beta 6(+) cells are recognized as PA-specific Treg cells. CD4(+)CD25(+)V beta 6(+) cells effectively suppressed the allo-reactive proliferation of lymphocytes compared with that of CD4(+)CD25(+)V beta 6(-) cells. V beta 6(+) PA-specific Treg cells expressed CCR4 and CCR5 on their surface. The frequency of Ki67(+) PA-specific Treg cells among Treg cells was significantly increased in draining lymph nodes on day 3.5 post-coitus (pc; 6.8 +/- 1.1%, p < 0.05) and day 5.5 pc (7.2 +/- 1.1%, p < 0.05) in allogeneic pregnant mice compared with that in nonpregnant mice (2.7 +/- 0.2%). The frequency of Ki67(+) PA-specific Treg cells in the uterus increased significantly after day 5.5 pc in allogeneic pregnant mice compared with that in nonpregnant mice (8.8 +/- 2.8% vs. 1.2 +/- 1.3%, p < 0.05). However, Ki67-PA-specific Tregs did not change during pregnancy. To analyze the role of seminal fluid or sperm in Treg expansion, female BALB/c mice were mated with vasectomized DBA/2 male mice (VAS) or seminal vesicle-excised DBA/2 male mice (SVX). The frequency of Ki67+ PA-specific Treg cells did not increase in draining lymph nodes or uterus in BALB/c x DBA/2 (SVX) allogeneic mating mice. These findings suggest that the priming by seminal fluid is important for the induction of proliferating PA-specific Tregs in uterine-draining lymph nodes just before implantation and pregnant uterus after implantation, resulting in successful implantation and the maintenance of allogeneic pregnancy. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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