期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 48, 期 6, 页码 3363-3371出版社
AMER CHEMICAL SOC
DOI: 10.1021/es4046567
关键词
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资金
- National Basic Research Program of China [2010CB923303]
- National High Technology Research and Development Program of China [2014AA020512]
- Jiangsu Natural Science Foundation [BK2011547]
- National Natural Science Foundation of China [91013009]
- Guangdong Government [S20120011226]
- Shenzhen Government [JC201104210113A, KQC201105310016A]
A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream RFP (red fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions. The subsequent display of PbrR on the E. coli cell surface permitted selective adsorption of lead ions from solution containing various heavy metal ions. The surface-engineered E. coli bacteria effectively protected Arabidopsis thaliana seed germination from the toxicity of lead ions at high concentrations. Engineering the E. colt bacteria harboring these lead-specific elements from the pbr operon may potentially be a valuable general strategy for biodetection and bioremediation of toxic heavy metal ions in the environment.
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