期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 48, 期 13, 页码 7511-7518出版社
AMER CHEMICAL SOC
DOI: 10.1021/es5012466
关键词
-
资金
- Environmental Security Technology Certification Program (ESTCP) [ER-200541]
- Consejo Nacional de Ciencia y Tecnologia (CONACYT)
We studied the microbial community structure of pilot two-stage membrane biofilm reactors (MBfRs) designed to reduce nitrate (NO3-) and perchlorate (ClO4-) in contaminated groundwater. The groundwater also contained oxygen (O-2) and sulfate (SO42-), which became important electron sinks that affected the NO3- and ClO4- removal rates. Using pyrosequencing, we elucidated how important phylotypes of each primary microbial group, i.e., denitrifying bacteria (DB), perchlorate-reducing bacteria (PRB), and sulfate-reducing bacteria (SRB), responded to changes in electron-acceptor loading. UniFrac, principal coordinate analysis (PCoA), and diversity analyses documented that the microbial community of biofilms sampled when the MBfRs had a high acceptor loading were phylogenetically distant from and less diverse than the microbial community of biofilm samples with lower acceptor loadings. Diminished acceptor loading led to SO42- reduction in the lag MBfR, which allowed Desulfovibrionales (an SRB) and Thiothrichales (sulfur-oxidizers) to thrive through S cycling. As a result of this cooperative relationship, they competed effectively with DB/PRB phylotypes such as Xanthomonadales and Rhodobacterales. Thus, pyrosequencing illustrated that while DB, PRB, and SRB responded predictably to changes in acceptor loading, a decrease in total acceptor loading led to important shifts within the primary groups, the onset of other members (e.g., Thiothrichales), and overall greater diversity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据