期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 44, 期 7, 页码 2721-2727出版社
AMER CHEMICAL SOC
DOI: 10.1021/es903043p
关键词
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资金
- J. Craig Venter Institute
- Legler-Benbough Foundation
- Office of Science (BER), US Department of Energy [DE-FG02-08ER64560]
Microbial fuel cell (MFC) technology has enabled new insights into the mechanisms of electron transfer from dissimilatory metal reducing bacteria to a solid phase electron acceptor. Using solid electrodes as electron acceptors enables quantitative real-time measurements of electron transfer rates to these surfaces. We describe here an optically accessible, dual anode, continuous flow MFC that enables real-time microscopic imaging of anode populations as they develop from single attached cells to a mature biofilms. We used this system to characterize how differences in external resistance affect cellular electron transfer rates on a per cell basis and overall biofilm development in Shewanella oneidensis strain MR-1. When a low external resistance (100 Q) was used, estimates of current per cell reached a maximum of 204 fA/cell (1.3 x 10(6) e(-) cell(-1) sec(-1)), while when a higher (1 M Omega) resistance was used, only 75 fA/cell (0.4 x 10(6) e(-) cell(-1) sec(-1)) was produced. The 1 M Omega anode biomass consistently developed into a mature thick biofilm with tower morphology (> 50 mu m thick), whereas only a thin biofilm (<5 mu m thick) was observed on the 100 Q anode. These data suggest a link between the ability of a surface to accept electrons and biofilm structure development.
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