期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 44, 期 23, 页码 8891-8896出版社
AMER CHEMICAL SOC
DOI: 10.1021/es103215b
关键词
-
资金
- National Natural Science Foundation of China [20777036, 40701073, 40971137, 21077056, 41071212]
- Natural Science Foundation of Jiangsu Province [BK2009315]
- Fok Ying Tung Education Foundation [122045]
- Foundation of the State Key Laboratory of Soil and Sustainable Agriculture, China [Y052010021]
A rapid inhibitory effect of polycyclic aromatic hydrocarbons (PAHs) on DNA degradation was examined by conventional spectral analysis and microtitration. The purpose was to determine whether PAHs inhibited free DNA degradation by the enzyme DNase I. The results showed that model PAHs phenanthrene and pyrene combined with free DNA to decelerate DNA degradation by DNase I. Phenanthrene-induced inhibition was stronger than that of pyrene. Trace level of PAHs did not induce DNase I deactivation. The DNase I enzyme exhibited only slight shifts in IR absorption bands related to amide II and III upon PAH exposure, and no :Mange was observed with other bands. The decelerating degradation of DNA is attributed to the changes in structure, backbone composition, and guanine constituents of DNA induced by PAHs inserted into double strands, and to the imidazole-like derivates from the combination of imidazole rings with pyrene.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据