期刊
ENVIRONMENTAL POLLUTION
卷 243, 期 -, 页码 670-678出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2018.09.040
关键词
Dicamba-based formulation Banvel (R); Long-term assay; 2,4-D-based formulation DMA (R); Sublethal effects; SCGE assay
资金
- National University of La Plata [11/N817, 11/N847]
- National Agency of Scientific and Technological Promotion from Argentina [3059]
Long-term genotoxic effects of two auxinic herbicide formulations, namely, the 58.4% 2,4-dichlorophenoxyacetic acid (2,4-D)-based DMA (R) and the 57.7% dicamba (DIC)-based Banvel (R) were evaluated on Cnesterodon decemmaculatus. Primary DNA lesions were analyzed by the single-cell gel electrophoresis methodology. Two sublethal concentrations were tested for each herbicide corresponding to 2.5% and 5% of the LC50(96h) values. Accordingly, fish were exposed to 25.2 and 50.4 mg/L or 41 and 82 mg/L for 2,4-D and DIC, respectively. Fish were continuously exposed for 28 days with replacement of test solutions every 3 days. Genotoxicity was evaluated in ten individuals from each experimental point at the beginning of the exposure period (0 day) and at 7, 14, 21 and 28 days thereafter. Results demonstrated for first time that 2,4-D-based formulation DMA (R) induced primary DNA strand breaks after 7-28 days exposure on C. decemmaculatus regardless its concentration. On the other hand, DIC-based formulation Banvel (R) exerted its genotoxic effect after exposure during 7-14 days and 7 days of 2.5 and 5% LC50(96h), respectively. The present study represents the first evidence of primary DNA lesions induced by two widely employed auxinic herbicides on C. decemmaculatus, namely 2,4-D and DIC, following long-term exposure. (C) 2018 Elsevier Ltd. All rights reserved.
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