期刊
ENVIRONMENTAL POLLUTION
卷 178, 期 -, 页码 419-425出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2013.03.057
关键词
Pyrosequencing; Ethanol; Microbial diversity; Temperature
资金
- American Petroleum Institute
- China Scholarship Council
The fate of fuel releases largely depends on the poorly-understood response in microbial community structure and function. Here, we evaluate the impacts to the microbial community resulting from a pilot-scale continuous release (10 months) of a 10% v:v ethanol solution mixed with benzene and toluene (50 mg/L each). Microbial population shifts were characterized by pyrosequencing-based 16S rRNA analysis and by quantitative PCR targeting Bacteria, Archaea, and functional genes for methanogenesis (mcrA), acetogenesis (fhs) and aerobic degradation of aromatic hydrocarbons (PHE), which could occur in hypoxic micro-environments. The release stimulated microbial growth, increased species richness and diversity, and selected for genotypes involved in fermentative degradation (the relative abundance of mcrA and fhs increased 18- and 6-fold, respectively). The growth of putative hydrocarbon degraders and commensal anaerobes, and increases in microbial diversity and in degradation rates suggest an adaptive response that increases the potential for natural attenuation of ethanol blend releases. (c) 2013 Elsevier Ltd. All rights reserved.
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