期刊
ENVIRONMENTAL POLLUTION
卷 159, 期 10, 页码 2808-2814出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2011.05.014
关键词
Microbial source tracking; (GTG)(5)-PCR; Fecal pollution; Shellfish growing water; Zoonotic pathogen
资金
- Zhejiang Gongshang University, China [1110XJ130919]
- Zhejiang Provincial Science and Technology Foundation, China [2009C33058]
This study was conducted to analyze the genetic variability of Escherichia coli from domesticated animal wastes for microbial source tracking (MST) application in fecal contaminated shellfish growing waters of Xiangshan Bay. East China Sea. (GTG)(5) primer was used to generate 1363 fingerprints from E. con isolated from feces of known 9 domesticated animal sources around this shellfish culture area. Jackknife analysis of the complete (GTG)(5)-PCR DNA fingerprint library indicated that isolates were assigned to the correct source groups with an 84.28% average rate of correct classification. Based on one-year source tracking data, the dominant sources of E. coli were swine, chickens, ducks and cows in this water area. Moreover, annual and spatial changes of E. coli concentrations and host sources may affect the level and distribution of zoonotic pathogen species in waters. Our findings will further contribute to preventing fecal pollution in aquatic environments and quality control of shellfish. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据