期刊
ENVIRONMENTAL MICROBIOLOGY
卷 17, 期 1, 页码 47-63出版社
WILEY
DOI: 10.1111/1462-2920.12395
关键词
-
类别
资金
- Ministerio de Economia y Competitividad (Madrid, Spain) [BIO2012-39695-C03-01]
- Ministerio de Ciencia e Innovacion (Madrid, Spain) [BFU-2009-11545-C03-01]
- Conserjeria de Educacion (Junta de Castilla y Leo, Valladolid, Spain) [LE246A11-2, LE114U13]
- Canadian Institutes for Health Research
- Ministerio de Ciencia e Innovacion
- Ministerio de Educacion y Ciencia
- Fonds de Recherche en Sante du Quebec
- Michael Smith Foundation for Health Research
Pseudomonas putidaDOC21, a soil-dwelling proteobacterium, catabolizes a variety of steroids and bile acids. Transposon mutagenesis and bioinformatics analyses identified four clusters of steroid degradation (std) genes encoding a single catabolic pathway. The latter includes three predicted acyl-CoA synthetases encoded by stdA1, stdA2 and stdA3 respectively. The stdA1 and stdA2 deletion mutants were unable to assimilate cholate or other bile acids but grew well on testosterone or 4-androstene-3,17-dione (AD). In contrast, a stdA3 mutant grew poorly in media containing either testosterone or AD. When cells were grown with succinate in the presence of cholate, stdA1 accumulated (1/4)-3-ketocholate and (1,4)-3-ketocholate, whereas stdA2 only accumulated 7,12-dihydroxy-3-oxopregna-1,4-diene-20-carboxylate (DHOPDC). When incubated with testosterone or bile acids, stdA3 accumulated 3a-H-4(3propanoate)-7a-methylhexahydro-1,5-indanedione (HIP) or the corresponding hydroxylated derivative. Biochemical analyses revealed that StdA1 converted cholate, 3-ketocholate, (1/4)-3-ketocholate, and (1,4)-3-ketocholate to their CoA thioesters, while StdA2 transformed DHOPDC to DHOPDC-CoA. In contrast, purified StdA3 catalysed the CoA thioesterification of HIP and its hydroxylated derivatives. Overall, StdA1, StdA2 and StdA3 are acyl-CoA synthetases required for the complete degradation of bile acids: StdA1 and StdA2 are involved in degrading the C-17 acyl chain, whereas StdA3 initiates degradation of the last two steroid rings. The study highlights differences in steroid catabolism between Proteobacteria and Actinobacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据