期刊
ENVIRONMENTAL MICROBIOLOGY
卷 12, 期 11, 页码 2977-2987出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1462-2920.2010.02274.x
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资金
- National Institute of General Medical Sciences [F32GM084640]
- Center for Plant and Microbial Complex Carbohydates [DE-FG09-93ER-20097]
- National Science Foundation
- Gordon and Betty Moore Foundation
- Department of Energy Genome to Life (GTL) programme
- Division Of Ocean Sciences
- Directorate For Geosciences [0911031] Funding Source: National Science Foundation
P>The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives.
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