DETERMINATION OF ORGANIC COMPOUNDS FROM DIFFERENT TYPES OF COFFEE BY HPLC AND GC-ECD ANALYSIS

A study was conducted to investigate the organic compounds residues in different types of coffee from market in Romania. High pressure liquid chromatography with fluorescence detector (HPLC), respectively gas chromatography with electron capture detection (GC-ECD) was used for the determination of 7 polycyclic aromatic hydrocarbons (PAHs) and 9 organochlorine pesticides (OCPs) in coffee samples. It was found that concentrations of PAHs in coffee samples varied from 0.001 mu g/Kg to 90.732 mu g/Kg. B[a]P was absent in the green coffee samples. The detection limits ranged from 0.02 ng/kg to 0.04 ng/kg and quantification limits were 0.2 ng/kg. Calibration and recovery studies gave satisfactory results. Concentrations of OCPs in coffee samples varied from 0.001 mg/Kg to 0.007 mg/Kg. The methods provide a rapid and accurate determination of these organic compounds in coffee samples.