期刊
ENVIRONMENTAL CHEMISTRY
卷 7, 期 3, 页码 309-317出版社
CSIRO PUBLISHING
DOI: 10.1071/EN10015
关键词
anodic stripping voltammetry; DOM; metal bioavailability
资金
- Spanish Government [CTM2006-13880-C03-01/MAR, CTM2009-10908]
- Spanish Ministry of Education and Science, Spanish Government
- Swiss National Science Foundation [PP022 118989]
Metal complexation by dissolved organic matter, as humic acids, is considered to decrease metal bioavailability by lowering the free metal ion concentration. However, dissolved organic matter adsorption on cell surfaces can modify cell membrane properties, which can also influence metal uptake. Copper and lead accumulation and internalisation by the marine diatom Thalassiosira weissflogii were studied in the absence and presence of humic acids, and adsorption of humic acids to cell surfaces was evaluated. Both Pb and Cu intracellular concentrations decreased in the presence of humic acids according to labile metal concentrations measured by anodic stripping voltammetry, whereas total (intracellular plus adsorbed) metal content was enhanced in the presence of humic acids, probably owing to enhanced metal plus humics adsorption to cell surfaces. The results of the present work stress the importance of differentiating between intracellular and total cellular metal in bioavailability studies, and suggest that the silica frustule of diatoms represents a barrier against humic acids effects on cell membranes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据