期刊
ENVIRONMENTAL CHEMISTRY
卷 7, 期 1, 页码 36-49出版社
CSIRO PUBLISHING
DOI: 10.1071/EN09125
关键词
coherent anti-Stokes Raman scattering; in vitro; metal oxides; rainbow trout; silver
资金
- Natural Environment Research Council [NER/S/A/2005/13319, NE/D004942/1, NE/C002369/1]
- UK Environment Agency
- NERC [NE/G011133/1, FENAC010001, NE/D004942/1] Funding Source: UKRI
- Natural Environment Research Council [NE/G011133/1, FENAC010001] Funding Source: researchfish
Relatively little is known regarding the fate and possible toxic effects of engineered nanoparticles (ENPs) in the aquatic environment. We assessed the suitability of isolated trout hepatocytes for high throughput toxicity screening of ENPs, exposing them to a variety of metal and metal oxide nanoparticles and their bulk counterparts. We found no effects of the ENPs on cell viability, or on lipid peroxidation, with the exception of exposure to ZnO nanoparticles, or on glutathione-S-transferase (GST) levels, for exposure concentrations up to 500 mu g mL(-1). All ENPs, however, were internalised in the cultured hepatocytes, as shown by coherent anti-Stokes Raman scattering (CARS) as an imaging technique. Our findings suggest that fish hepatocyte cultures are suitable for studies investigating the cellular uptake of ENPs, but they do not appear to be sensitive to ENP exposure and thus not a good in vitro model for nanoparticle toxicity screening.
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