期刊
ENVIRONMENTAL BIOLOGY OF FISHES
卷 91, 期 2, 页码 243-249出版社
SPRINGER
DOI: 10.1007/s10641-011-9766-6
关键词
Coilia ectenes; Coilia mystus; Coilia nasus; Microsatellites markers; Cross-species amplification; Genetic structure
资金
- Major State Basic Research Development Program of China (973 Program) [2010CB429005]
- East China Sea Fisheries Research Institute [2008Z02, 2008M04]
- National Natural Science Foundation of China [30600456]
- China Postdoctoral Science Foundation [20060400622]
- Commission of Science and Technology, Shanghai, China [08391910300]
- Keji Xing Nong, Shanghai, China [2008-7-2]
In this study, twelve polymorphic microsatellite markers were developed for Coilia ectenes (synonym C. nasus). In a sample of 30 C. ectenes individuals in a Wuhan population from Yangtze River, a total of 78 alleles were detected, and the number of alleles per locus ranged from 3 to 12. The observed and expected heterozygosities ranged from 0.100 to 1.000 and from 0.098 to 0.899, respectively. Six loci significantly deviated from the Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.004), and no significant linkage disequilibrium between pairs of loci was found. The applicability of these markers in a closely related species, C. mystus, was evaluated by cross-species amplification. These microsatellite markers will be useful in studies on population genetics, conservation genetics, and fishery management and in the construction of genetic linkage maps of C. ectenes and C. mystus.
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