期刊
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
卷 50, 期 1, 页码 37-45出版社
WILEY
DOI: 10.1002/em.20435
关键词
DNA damage; pesticide sprayers; comet assay; HPLC
A cross-sectional study was designed to determine whether occupational exposure to a complex mixture of pesticides results in a significant increase of DNA damage in farmers chronically exposed to pesticides in open fields. Leukocytes from 47 agriculture workers exposed to pesticides and 50 controls were evaluated with comet assay. Workers recruitment was based on their exposure to pesticides during the spraying season on cotton crop. Serum from these individuals was also analyzed for pesticides presence using high performance liquid chromatography. Statistically significant difference (P < 0.001) in DNA damage of exposed individuals (mean +/- S.D 14.80 +/- 3.04 mu m) was observed when compared with control group (6.54 +/- 1.73 mu m) as studied on the basis of comet tail length. Smokers had significantly higher mean comet tail length than nonsmokers and ex-smokers in both workers (20.26 +/- 3.53 vs. 14.19 +/- 4.25, P < 0.001) and controls (7.86 +/- 1.09 vs. 5.80 +/- 1.59, P < 0.001), whereas age had a minimal effect on DNA damage (P < 0.05). The length of pesticide exposure is positively associated with DNA damage in exposed individuals (P < 0.001). Our study shows that chronic exposure to pesticides produces DNA damage in pesticide sprayers and suggests that this type of monitoring is recommended in preventive policies for pesticide sprayers. Environ. Mal. Mutagen. 50:37-45, 2009, (C) 2008 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据