期刊
ENVIRONMENT INTERNATIONAL
卷 35, 期 3, 页码 588-593出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2008.11.003
关键词
PBDD/F; PCDD/F; PBCDD/F; CALUX; ELISA; Dioxin-like activity
资金
- U.S. EPA, Research Triangle Park, NC
- Xenobiotic Detection Systems, Durham, NC
Determination of toxic activity requires knowledge of both the concentration and toxicity to evaluate the risk for adverse human health and environmental effects. A chemically-activated luciferase gene expression cell bioassay system (CALUX) and an antibody-based method enzyme immunoassay (EIA) were used to detect the dioxin-like response of several polybrominated, polychlorinated, and polybrominated/chlorinated dibenzo-p-dioxins/furans (PBDDs/Fs, PCDDs/Fs, and PBCDDs/Fs, respectively). It has been suggested that the biological activity of the brominated and mixed bromo/chloro compounds is similar to their chlorinated analogues (measured by binding to the Ah receptor). PBDD/F, PCDD/F, and PBCDD/F laboratory standards exhibited biological activity ranging over three orders of magnitude. The highest relative potency (REP) values from CALUX analysis, when compared to 2,3,7,8-TCDD, were 2,3,7,8-TBDD at 0.99 (+/- 0.07), 1,2,3,7,8=PeCDD at 0.69, and 2-Br-3,7,8-TriCDD at 0.72 (+/- 0.02). Cross-reactivities were calculated using EIA for several PBDDs/Fs and PBCDDs. The highest percent cross-reactivity was found for 2,3,7,8-TBDD at 138 (+/- 34%), and 2,3,7-TriBDD at 84 (+/- 36%). Published by Elsevier Ltd.
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