期刊
JOURNAL OF PROTEOMICS
卷 123, 期 -, 页码 1-13出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2015.03.035
关键词
Clostridium difficile; Spores; Exosporium; Spore surface proteins
资金
- Fonda Nacional de Ciencia y Tecnologia de Chile (FONDECYT) [1110569]
- Research Office of Universidad Andres Bello [DI-275-13/R 2013]
- Fonda de Fomento al Desarrollo Cientifico y Tecnologico (FONDEF) [CA13I10077]
- U.S. Army Research Office [W911NF-09-1-0286]
- NIH/NIEHS [P30 ES000210]
- N.L. Tartar Foundation of Oregon State University
- Agricultural Research Foundation of Oregon State University
- Department of Defense Multi-disciplinary University Research Initiative (MURI) award through the U.S. Army Research Laboratory
Clostridium difficile spores are considered the morphotype of infection, transmission and persistence of C. difficile infections. There is a lack of information on the composition of the outermost exosporium layer of C. difficile spores. Using recently developed exosporium removal methods combined with MS/MS, we have established a gel-free approach to analyze the proteome of the exosporium of C. difficile spores of strain 630. A total of 184 proteins were found in the exosporium layer of C. difficile spores. We identified 7 characterized spore coat and/or exosporium proteins; 6 proteins likely to be involved in spore resistance; 6 proteins possibly involved in pathogenicity; 13 uncharacterized proteins; and 146 cytosolic proteins that might have been encased into the exosporium during assembly, similarly as reported for Bacillus anthracis and Bacillus cereus spores. We demonstrate through Flag-fusions that CotA and CotB are mainly located in the spore coat, while the exosporium collagen-like glycoproteins (i.e. Bc1A1, Bc1A2 and Bc1A3), the exosporium morphogenetic proteins CdeC and CdeM, and the uncharacterized exosporium proteins CdeA and CdeB are mainly located in the exosporium layer of C. difficile 630 spores. This study offers novel candidates of C. difficile exosporium proteins as suitable targets for detection, removal and spore-based therapies. Biological significance This study offers a novel strategy to identify proteins of the exosporium layer of C. difficile spores and complements previous proteomic studies on the entire C. difficile spores and spore coat since it defines the proteome of the outermost layer of C. difficile spores, the exosporium. This study suggests that C. difficile spores have several proteins involved in protection against environmental stress as well as putative virulence factors that might play a role during infection. Spore exosporium structural proteins were also identified providing the ground basis for further functional studies of these proteins. Overall this work provides new protein target for the diagnosis and/or therapeutics that may contribute to combat C. difficile infections. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据