期刊
ENGINEERING IN LIFE SCIENCES
卷 14, 期 2, 页码 143-152出版社
WILEY
DOI: 10.1002/elsc.201200220
关键词
Fluorescence in situ hybridization (FISH); Mixed microbial cultures (MMC); pH control; Phosphorus; Polyhydroxyalkanoates (PHA)
资金
- Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [PTDC/EBB-EBI/103147/2008, PTDC/AGR-ALI/122741/2010, PEst-C/EQB/LA0006/2011]
- EU through the project Ecoefficient Biodegradable Composite Advanced Packaging (EcoBioCAP) [0265669]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-ALI/122741/2010] Funding Source: FCT
Polyhydroxyalkanoate (PHA) production via mixed microbial cultures (MMCs) can potentially decrease process operational costs as compared to conventional pure culture techniques. However, the volumetric productivity of PHA by MMCs must be augmented to increase its cost competitiveness. For this purpose, a three-stage bioreactor system was operated in this study, with (i) anaerobic fermentation of molasses, (ii) culture selection, and (iii) PHA accumulation and harvesting stages. In stage2, bioreactor operation with pH control at 8 led to twice the biomass concentration (up to 8 g VSS L-1, where VSS is the volatile suspended solids) as compared to operation without pH control (maximum pH 9). No loss in the specific PHA storage efficiency was observed (PHA content up to 57.5% and PHA storage rate up to 0.27 Cmol PHA Cmol X-1 h(-1), where X is the active biomass), thereby resulting in twice the volumetric PHA production rate. The limited biomass growth at the higher pH level was not due to nutrient limitation, but likely to a shift in the microbial community. It is hypothesized that the increased enrichment of Azoarcus at pH 8 led to higher PHA productivity. pH control in the culture selection stage can lead to enhanced PHA production from MMCs, improving the viability of the process.
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