期刊
ENGINEERING IN LIFE SCIENCES
卷 11, 期 2, 页码 182-188出版社
WILEY
DOI: 10.1002/elsc.201000068
关键词
Biosensors; Enzyme assay; Glucose oxidase; Laccase; Microfluidic devices
The commonly used assay for measuring glucose oxidase (GOx) and laccase (Lac) activity, based on spectrophotometric test using 2,2'-azinobis-(3-ethylbenzothia-zoline-6-sulfonic acid) (ABTS), has been adapted to measure these enzyme activities via microfluidic devices. The effects of flow rate and concentration of substrates were examined. The developed assays allowed analyzing GOx and Lac activities in the range of 0.8-8.0 Unit (0.1-1.0 mg/mL) and 0.9-17.7 Unit (0.1-2.0 mg/mL), respectively. Moreover, repeatability tests were obtained for both enzyme assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据