期刊
ENGINEERING IN LIFE SCIENCES
卷 10, 期 1, 页码 57-64出版社
WILEY
DOI: 10.1002/elsc.200900001
关键词
Acetylcholinesterase; Electrospinning; Immobilization; Nanofiber; PVA
资金
- Research Center of Basic Science, Shahed University
Electrospinning, a simple and versatile method to fabricate nanofibrous supports, has attracted continuous attention in the field of enzyme immobilization. In this study, acetylcholinesterase (AChE) has been successfully immobilized in PVA nanofibers via electrospinning of a mixture of AChE, BSA as an enzyme stabilizing additive and PVA. The maximum activity recovery of immobilized AChE was about 40%. In comparison with free enzyme, the immobilized AChE showed improved stability while retaining a considerable amount of activity at lower pH values. Moreover, the immobilized AChE retained > 34% of its initial activity when stored at 30 degrees C for 100 days and retained 70% of its initial activity after ten consecutive reactor batch cycles.
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