期刊
ENDOCRINOLOGY
卷 150, 期 6, 页码 2740-2748出版社
ENDOCRINE SOC
DOI: 10.1210/en.2008-1536
关键词
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资金
- Canadian Institutes of Health Research (CIHR) [MOP-10369]
- University of Ottawa International Creative Research Initiatives [100146]
- Program on Oocyte Health
- Healthy Gametes and Great Embryos Strategic Initiative of CIHR Institute of Human Development, Child and Youth Health [HGG62293]
- Ministry of Education, Culture, Sports, Science, and Technology, Japan
- CIHR Strategic Training Initiatives in the Research in Reproductive Sciences
- Grants-in-Aid for Scientific Research [21592093] Funding Source: KAKEN
The transition from preantral to early antral stage is the penultimate stage of ovarian follicular development in terms of gonadotropin dependence and follicle destiny. Although oocyte-somatic cell communication is important in early follicular development, our knowledge of the precise role of the oocyte-derived growth differentiation factor (GDF)-9 during preantral follicle growth is incomplete. We examined whether and by what means oocyte-derived GDF-9 controls follicular development and steroidogenesis during the preantral to early antral transition, by a combination of in vitro gene manipulation (i.e. intraoocyte injection of GDF-9 antisense oligos) and preantral follicle culture. Intraoocyte injection of GDF-9 antisense suppressed rat preantral follicle growth in vitro, whereas GDF-9 enhanced follicular development. GDF-9 augmented testosterone production in preantral follicles. GDF-9 antisense suppressed androgen production and CYP17A1 mRNA expression in cultured follicles, a response attenuated by exogenous GDF-9. The nonaromatizable androgen 5 alpha-dihydrotestosterone rescued the follicular growth arrest caused by GDF-9 down-regulation. The specific androgen receptor antagonist flutamide suppressed GDF-9-induced preantral follicle growth in vitro. The data suggest that GDF-9 plays an important role in promoting preantral follicle growth by up-regulating follicular androgen biosynthesis. GDF-9 is essential for CYP17A1 expression during follicular development from the preantral to the early antral stage. (Endocrinology 150: 2740-2748, 2009)
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