期刊
EMBO REPORTS
卷 15, 期 2, 页码 142-154出版社
WILEY
DOI: 10.1002/embr.201338166
关键词
RBR; E3 ligase; TRIAD; autoinhibition; ubiquitination; mechanism
资金
- ERC advanced grant 'Ubiquitin balance' [24997]
The RING-in-between-RING (RBR) E3s are a curious family of ubiquitin E3-ligases, whose mechanism of action is unusual in several ways. Their activities are auto-inhibited, causing a requirement for activation by protein-protein interactions or posttranslational modifications. They catalyse ubiquitin conjugation by a concerted RING/HECT-like mechanism in which the RING1 domain facilitates E2-discharge to directly form a thioester intermediate with a cysteine in RING2. This short-lived, HECT-like intermediate then modifies the target. Uniquely, the RBR ligase HOIP makes use of this mechanism to target the ubiquitin amino-terminus, by presenting the target ubiquitin for modification using its distinctive LDD region.
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