期刊
EMBO REPORTS
卷 14, 期 12, 页码 1084-1091出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2013.164
关键词
chromatin; H2A.Z; immediate early genes; MAP kinase signalling; PARP
资金
- Wellcome Trust
- Cancer Research UK
- Royal Society-Wolfson award
- Cancer Research UK [13220] Funding Source: researchfish
Transcriptional activation is accompanied by multiple molecular events that remodel the local chromatin environment in promoter regions. These molecular events are often orchestrated in response to the activation of signalling pathways, as exemplified by the response of immediate early genes such as FOS to ERK MAP kinase signalling. Here, we demonstrate that inducible NFI recruitment permits PARP1 binding to the FOS promoter by a mutually reinforcing loop. PARP1 and its poly(ADP-ribosyl)ation activity are required for maintaining FOS activation kinetics. We also show that the histone variant H2A.Z associates with the FOS promoter and acts in a transcription-suppressive manner. However, in response to ERK pathway signalling, H2A.Z is replaced by H2A; PARP1 activity is required to promote this exchange. Thus, our work has revealed an additional facet of PARP1 function in promoting dynamic remodelling of promoter-associated nucleosomes to allow transcriptional activation in response to cellular signalling.
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