期刊
EMBO REPORTS
卷 14, 期 12, 页码 1098-1103出版社
WILEY
DOI: 10.1038/embor.2013.158
关键词
Chd1; chromatin remodeller; nucleosome sliding
资金
- National Institutes of Health [R01-GM084192]
Chromatin remodellers are ATP-dependent motor proteins that physically reposition and reorganize nucleosomes. Chd1 and Iswi-type remodellers possess a DNA-binding domain (DBD) needed for efficient nucleosome mobilization; however, it has not been clear how this domain physically contributes to remodelling. Here we show that the Chd1 DBD promotes nucleosome sliding simply by tethering the remodeller to nucleosome substrates. Nucleosome sliding activity was largely resistant to increasing length and flexibility of the linker connecting the DBD and ATPase motor, arguing that the ATPase motor does not shift DNA onto the nucleosome by pulling on the DBD.
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