期刊
EMBO REPORTS
卷 13, 期 11, 页码 992-996出版社
WILEY
DOI: 10.1038/embor.2012.139
关键词
chromatin fibre; nuclear organization; electron tomography
资金
- Canadian Institutes of Health Research [MOP-111153, MOP-111065, IG1-102956]
- Natural Sciences and Engineering Research Council
The mammalian genome is compacted to fit within the confines of the cell nucleus. DNA is wrapped around nucleosomes, forming the classic 'beads-on-a-string' 10-nm chromatin fibre. Ten-nanometre chromatin fibres are thought to condense into 30-nm fibres. This structural reorganization is widely assumed to correspond to transitions between active and repressed chromatin, thereby representing a chief regulatory event. Here, by combining electron spectroscopic imaging with tomography, three-dimensional images are generated, revealing that both open and closed chromatin domains in mouse somatic cells comprise 10-nm fibres. These findings indicate that the 30-nm chromatin model does not reflect the true regulatory structure in vivo.
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