期刊
EMBO REPORTS
卷 13, 期 12, 页码 1130-1137出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2012.161
关键词
DNA replication; mitochondrion; origin; primer; TWINKLE
资金
- Swedish Research Council
- Swedish Cancer Foundation
- Leducq Foundation
- European Research Council
- Swedish Society for Medical Research
- European Commission
The mechanisms of mitochondrial DNA replication have been hotly debated for a decade. The strand-displacement model states that lagging-strand DNA synthesis is initiated from the origin of light-strand DNA replication (OriL), whereas the strand-coupled model implies that OriL is dispensable. Mammalian mitochondria cannot be transfected and the requirements of OriL in vivo have therefore not been addressed. We here use in vivo saturation mutagenesis to demonstrate that OriL is essential for mtDNA maintenance in the mouse. Biochemical and bioinformatic analyses show that OriL is functionally conserved in vertebrates. Our findings strongly support the strand-displacement model for mtDNA replication.
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