期刊
EMBO REPORTS
卷 12, 期 10, 页码 1055-1061出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2011.175
关键词
endocytosis; LGR4; LGR5; R-spondin; Wnt
资金
- Deutsche Forschungsgemeinschaft (DFG)
R-spondins are secreted Wnt signalling agonists, which regulate embryonic patterning and stem cell proliferation, but whose mechanism of action is poorly understood. Here we show that R-spondins bind to the orphan G-protein-coupled receptors LGR4 and LGR5 by their Furin domains. Gain-and loss-of-function experiments in mammalian cells and Xenopus embryos indicate that LGR4 and LGR5 promote R-spondin-mediated Wnt/beta-catenin and Wnt/PCP signalling. R-spondin-triggered beta-catenin signalling requires Clathrin, while Wnt3a-mediated beta-catenin signalling requires Caveolin-mediated endocytosis, suggesting that internalization has a mechanistic role in R-spondin signalling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据