期刊
EMBO REPORTS
卷 12, 期 10, 页码 1032-1038出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2011.158
关键词
anaphase bridge; PICH; common fragile site; lac operon; micronucleus
资金
- Spanish Ministry of Science [CSD2007-00017, SAF2008-01596]
- EMBO
- European Research Council [ERC-210520]
One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site through the cell cycle. By using this system, we show that Plk1-interacting checkpoint helicase (PICH) is already present at fragile sites during interphase, suggesting roles for this helicase beyond mitosis. In addition, we report that the oncogene Myc promotes the formation of anaphase bridges and micronuclei containing fragile-site sequences.
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