期刊
EMBO REPORTS
卷 12, 期 12, 页码 1251-1256出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2011.209
关键词
bimolecular fluorescence complementation; co-receptor; T-cell activation
资金
- National Institutes of Health (NIH) [R01GM065230, R01AI074074, T32HL07195]
- Irvington Institute of the Cancer Research Institute
Bimolecular fluorescence complementation was used to engineer CD8 molecules so that CD8 alpha alpha and CD8 alpha beta dimers can be independently visualized on the surface of a T cell during antigen recognition. Using this approach, we show that CD8aa is recruited to the immunological synapse almost as well as CD8ab, but because the kinase Lck associates preferentially with CD8ab in lipid rafts, CD8 alpha alpha is the weaker co-receptor. During recognition of the strong CD8 alpha alpha ligand H2-TL, CD8 alpha alpha is preferentially recruited. Thus, recruitment of the two CD8 species correlates with their relative binding to the available ligands, rather than with the co-receptor functions of the CD8 species.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据