期刊
EMBO REPORTS
卷 12, 期 8, 页码 797-803出版社
WILEY
DOI: 10.1038/embor.2011.101
关键词
Ash2L; histone methyltransferase; MLL; transcription; winged helix motif
资金
- National Institutes of Health [RO1 GM083015]
- American Cancer Society
Ash2L is a core component of the MLL family histone methyltransferases and has an important role in regulating the methylation of histone H3 on lysine 4. Here, we report the crystal structure of the N-terminal domain of Ash2L and reveal a new function of Ash2L. The structure shows that Ash2L contains an atypical PHD finger that does not have histone tail-binding activity. Unexpectedly, the structure shows a previously unrecognized winged-helix motif that directly binds to DNA. The DNA-binding-deficient mutants of Ash2L reduced Ash2L localization to the HOX locus. Strikingly, a single mutation in Ash2L(WH) (K131A) breaks the chromatin domain boundary, suggesting that Ash2L also has a role in chromosome demarcation.
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