期刊
EMBO REPORTS
卷 11, 期 9, 页码 705-710出版社
WILEY
DOI: 10.1038/embor.2010.113
关键词
nucleosome; RNA polymerase II; transcript elongation
资金
- National Science Foundation [0743298]
- National Institutes of Health [GM58650]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0743298] Funding Source: National Science Foundation
Nucleosome traversal by RNA polymerase II (pol II) and recovery of chromatin structure after transcription are essential for proper gene expression. In this paper we show that nucleosomes assembled with Sin mutant histones present a much weaker barrier to traversal by pol II and are less likely to survive transcription. Increases in traversal from incorporation of Sin mutant histones and histones lacking H2A/H2B amino-terminal tails were in most cases additive, indicating that traversal can be facilitated by distinct mechanisms. We had identified a key intermediate in traversal, the zero (empty set)-loop, which mediates nucleosome survival during transcription. Sin mutations probably destabilize these intermediates and thus increase the likelihood of nucleosome disassociation.
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