期刊
EMBO REPORTS
卷 11, 期 2, 页码 106-111出版社
WILEY
DOI: 10.1038/embor.2009.271
关键词
actinomycin D; exosome; non-canonical poly(A) polymerase; ribosomal RNA; RNA processing
资金
- National Institutes of Health [GM074091]
Most transcripts in growing cells are ribosomal RNA precursors (pre-rRNA). Here, we show that in mammals, aberrant pre-rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full-length rRNA. The poly(A) polymerase-associated domain-containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short aberrant transcripts. Thus, polyadenylation functions in the quality control of Pol I transcription in metazoan cells. The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD.
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