期刊
EMBO REPORTS
卷 11, 期 2, 页码 126-132出版社
WILEY
DOI: 10.1038/embor.2009.274
关键词
allelic exclusion; antigen-receptor gene; gene assembly; variable lymphocyte receptor
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20017005, 20055005]
- Japan Society for the Promotion of Science [20370067, 21659119]
- Yamada Science Foundation
- Grants-in-Aid for Scientific Research [20017005, 20055005, 21659119, 20370067] Funding Source: KAKEN
Variable lymphocyte receptors (VLRs) are antigen receptors in the jawless vertebrates lamprey and hagfish. VLR genes are classified into VLRA and VLRB, and lymphocytes expressing VLRA are T-cell-like, whereas those expressing VLRB are B-cell-like in the sea lamprey. Diverse VLR genes are assembled somatically in lymphocytes; however, how the assembly is regulated is still largely unknown. Here, we analyse VLR gene assembly at the single-cell level in the inshore hagfish (Eptatretus burgeri). Each lymphocyte assembles and transcribes only one type of VLR gene, either VLRA or VLRB. In general, monoallelic assembly of VLR was observed, but diallelic assembly was found in some cases-in many of which, one allele was functional and the other was defective. In fact, all VLR-assembled lymphocytes contained at least one functional VLR gene. Together, these results indicate a feedback inhibition of VLR assembly and selection of VLR-positive lymphocytes.
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