期刊
EMBO REPORTS
卷 10, 期 6, 页码 649-654出版社
WILEY
DOI: 10.1038/embor.2009.31
关键词
Arabidopsis; AGO4; SPT5; RdDM; silencing
资金
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche [ANR-08-BLAN-0206-01]
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0206] Funding Source: Agence Nationale de la Recherche (ANR)
Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxyterminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM.
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