期刊
EMBO REPORTS
卷 10, 期 2, 页码 160-165出版社
WILEY
DOI: 10.1038/embor.2008.243
关键词
ribosome; decoding; trans-translation; tmRNA; SmpB
资金
- Region Bretagne (PRIR [691]
- CRB [2004-1483]
- Action Concertee Incitative [BCMS 136]
- Agence Nationale de la Recherche programme [MIME 2006]
Tight recognition of codon-anticodon pairings by the ribosome ensures the accuracy and fidelity of protein synthesis. In eubacteria, translational surveillance and ribosome rescue are performed by the 'tmRNA-SmpB' system (transfer messenger RNA-small protein B). Remarkably, entry and accommodation of aminoacylated-tmRNA into stalled ribosomes occur without a codon-anticodon interaction but in the presence of SmpB. Here, we show that within a stalled ribosome, SmpB interacts with the three universally conserved bases G530, A1492 and A1493 that form the 30S subunit decoding centre, in which canonical codon anticodon pairing occurs. The footprints at positions A1492 and A1493 of a small decoding centre, as well as on a set of conserved SmpB amino acids, were identified by nuclear magnetic resonance. Mutants at these residues display the same growth defects as for Delta smpB strains. The SmpB protein has functional and structural similarities with initiation factor 1, and is proposed to be a functional mimic of the pairing between a codon and an anticodon.
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