期刊
EMBO REPORTS
卷 9, 期 3, 页码 267-272出版社
WILEY
DOI: 10.1038/embor.2008.1
关键词
histone code; RNA polymerase II; HIV1; mitogen-activated protein kinases
The mammalian heterochromatin protein 1 (HP1) family of proteins was recently shown to be involved in transient repression of inducible promoters. One of these promoters is the HIV1 long terminal repeat, which, during viral latency, recruits a non-processive RNA polymerase II (RNAPII) that synthesizes a short regulatory transcript. Here, we have used this promoter to examine the interplay of HP1 alpha, HP1 beta and HP1 gamma with RNAPII. We find that, in the absence of stimulation, HP1b is present on the promoter together with the non-processive RNAPII and functions as a negative regulator. On activation, HP1b bound to methylated H3K9 is rapidly released concurrent with histone H3 phospho-acetylation, and is replaced by HP1 gamma. This isoform localizes to the promoter but also inside the coding region, together with the processive RNAPII. Our data show that HP1 recruitment-release is a sequential mechanism that is precisely regulated and highly dependent on transcription.
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