期刊
EMBO MOLECULAR MEDICINE
卷 5, 期 6, 页码 904-918出版社
WILEY-BLACKWELL
DOI: 10.1002/emmm.201201429
关键词
calcium; endoplasmic reticulum; mitochondria; oxidative stress; Wolfram Syndrome
资金
- NIH [DK18024, DK018849, DK054441, DK081298, R3749512, RR004050]
- American Diabetes Association [1-08-RA-139]
- Ellison Medical Foundation [AG-SS-2190-08]
- Ontario Ministry of Health and Long Term Care
- Princess Margaret Hospital Foundation
Miner1 is a redox-active 2Fe2S cluster protein. Mutations in Miner1 result in Wolfram Syndrome, a metabolic disease associated with diabetes, blindness, deafness, and a shortened lifespan. Embryonic fibroblasts from Miner1-/- mice displayed ER stress and showed hallmarks of the unfolded protein response. In addition, loss of Miner1 caused a depletion of ER Ca2+ stores, a dramatic increase in mitochondrial Ca2+ load, increased reactive oxygen and nitrogen species, an increase in the GSSG/GSH and NAD+/NADH ratios, and an increase in the ADP/ATP ratio consistent with enhanced ATP utilization. Furthermore, mitochondria in fibroblasts lacking Miner1 displayed ultrastructural alterations, such as increased cristae density and punctate morphology, and an increase in O2 consumption. Treatment with the sulphydryl anti-oxidant N-acetylcysteine reversed the abnormalities in the Miner1 deficient cells, suggesting that sulphydryl reducing agents should be explored as a treatment for this rare genetic disease.
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