期刊
EMBO JOURNAL
卷 37, 期 19, 页码 -出版社
WILEY
DOI: 10.15252/embj.201899021
关键词
DNA polymerase alpha; DNA replication; histone chaperone; histones; replisome
资金
- Wellcome Trust [102943/Z/13/Z, 104641/Z/14/Z, 097945/B/11/Z]
- Medical Research Council [MC_UU_12016/13]
- MRC [MC_UU_00018/4, MC_UU_12016/13] Funding Source: UKRI
The eukaryotic replisome disassembles parental chromatin at DNA replication forks, but then plays a poorly understood role in the re-deposition of the displaced histone complexes onto nascent DNA. Here, we show that yeast DNA polymerase contains a histone-binding motif that is conserved in human Pol alpha and is specific for histones H2A and H2B. Mutation of this motif in budding yeast cells does not affect DNA synthesis, but instead abrogates gene silencing at telomeres and mating-type loci. Similar phenotypes are produced not only by mutations that displace Pol alpha from the replisome, but also by mutation of the previously identified histone-binding motif in the CMG helicase subunit Mcm2, the human orthologue of which was shown to bind to histones H3 and H4. We show that chromatin-derived histone complexes can be bound simultaneously by Mcm2, Pol alpha and the histone chaperone FACT that is also a replisome component. These findings indicate that replisome assembly unites multiple histone-binding activities, which jointly process parental histones to help preserve silent chromatin during the process of chromosome duplication.
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