期刊
EMBO JOURNAL
卷 34, 期 2, 页码 154-168出版社
WILEY-BLACKWELL
DOI: 10.15252/embj.201489083
关键词
autophagy; metabolome analysis; ribosome; RNA degradation; vacuole
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- JSPS
- Grants-in-Aid for Scientific Research [23000015, 13J40059] Funding Source: KAKEN
Autophagy is a catabolic process conserved among eukaryotes. Under nutrient starvation, a portion of the cytoplasm is non-selectively sequestered into autophagosomes. Consequently, ribosomes are delivered to the vacuole/lysosome for destruction, but the precise mechanism of autophagic RNA degradation and its physiological implications for cellular metabolism remain unknown. We characterized autophagy-dependent RNA catabolism using a combination of metabolome and molecular biological analyses in yeast. RNA delivered to the vacuole was processed by Rny1, a T2-type ribonuclease, generating 3-NMPs that were immediately converted to nucleosides by the vacuolar non-specific phosphatase Pho8. In the cytoplasm, these nucleosides were broken down by the nucleosidases Pnp1 and Urh1. Most of the resultant bases were not re-assimilated, but excreted from the cell. Bulk non-selective autophagy causes drastic perturbation of metabolism, which must be minimized to maintain intracellular homeostasis.
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