期刊
EMBO JOURNAL
卷 33, 期 18, 页码 1999-2019出版社
WILEY
DOI: 10.15252/embj.201386855
关键词
germ cell; maelstrom; mouse; piRNA; Piwi
资金
- Carnegie Institution for Science
- Searle Scholars Program
- National Institute of General Medical Studies of the National Institutes of Health [P41 GM103533]
Pachytene piRNAs are a class of Piwi-interacting small RNAs abundant in spermatids of the adult mouse testis. They are processed from piRNA primary transcripts by a poorly understood mechanism and, unlike fetal transposon-derived piRNAs, lack complementary targets in the spermatid transcriptome. We report that immunopurified complexes of a conserved piRNA pathway protein Maelstrom (MAEL) are enriched in MIWI (Piwi partner of pachytene piRNAs), Tudor-domain proteins and processing intermediates of pachytene piRNA primary transcripts. We provide evidence of functional significance of these complexes in Mael(129) knockout mice that exhibit spermiogenic arrest with acrosome and flagellum malformation. Mael(129)-null mutant testes possess low levels of piRNAs derived from MAEL-associated piRNA precursors and exhibit reduced translation of numerous spermiogenic mRNAs including those encoding acrosome and flagellum proteins. These translation defects in haploid round spermatids are likely indirect, as neither MAEL nor piRNA precursors associate with polyribosomes, and they may arise from an imbalance between pachytene piRNAs and MIWI.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据