期刊
EMBO JOURNAL
卷 33, 期 22, 页码 2606-2622出版社
WILEY-BLACKWELL
DOI: 10.15252/embj.201489244
关键词
CBX3; colon; Heterochromatin protein 1; MSK1; Shigella
资金
- Agence National de la Recherche (ANR-MIME)
- fondation pour la recherche medicale (FRM)
HP1 proteins are transcriptional regulators that, like histones, are targets for post-translational modifications defining an HP1-mediated subcode. HP1 gamma has multiple phosphorylation sites, including serine 83 (S83) that marks it to sites of active transcription. In a guinea pig model for Shigella enterocolitis, we observed that the defective type III secretion mxiD Shigella flexneri strain caused more HP1 gamma phosphorylation in the colon than the wild-type strain. Shigella interferes with HP1 phosphorylation by injecting the phospholyase OspF. This effector interacts with HP1 gamma and alters its phosphorylation at S83 by inactivating ERK and consequently MSK1, a downstream kinase. MSK1 that here arises as a novel HP1 gamma kinase, phosphorylates HP1 gamma at S83 in the context of an MSK1-HP1 gamma complex, and thereby favors its accumulation on its target genes. Genome-wide transcriptome analysis reveals that this mechanism is linked to up-regulation of proliferative gene and fine-tuning of immune gene expression. Thus, in addition to histones, bacteria control host transcription by modulating the activity of HP1 proteins, with potential implications in transcriptional reprogramming at the mucosal barrier.
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