期刊
EMBO JOURNAL
卷 31, 期 14, 页码 3169-3182出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2012.165
关键词
glutathione; Mia40; mitochondrial intermembrane space; porin; roGFP
资金
- Deutsche Forschungsgemeinschaft
- Landesschwerpunkt Membrantransport
- EMBO Long Term Fellowship
Glutathione is an important mediator and regulator of cellular redox processes. Detailed knowledge of local glutathione redox potential (E-GSH) dynamics is critical to understand the network of redox processes and their influence on cellular function. Using dynamic oxidant recovery assays together with E-GSH-specific fluorescent reporters, we investigate the glutathione pools of the cytosol, mitochondrial matrix and intermembrane space (IMS). We demonstrate that the glutathione pools of IMS and cytosol are dynamically interconnected via porins. In contrast, no appreciable communication was observed between the glutathione pools of the IMS and matrix. By modulating redox pathways in the cytosol and IMS, we find that the cytosolic glutathione reductase system is the major determinant of E-GSH in the IMS, thus explaining a steady-state E-GSH in the IMS which is similar to the cytosol. Moreover, we show that the local E-GSH contributes to the partially reduced redox state of the IMS oxidoreductase Mia40 in vivo. Taken together, we provide a comprehensive mechanistic picture of the IMS redox milieu and define the redox influences on Mia40 in living cells. The EMBO Journal (2012) 31, 3169-3182. doi:10.1038/emboj.2012.165; Published online 15 June 2012
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