期刊
EMBO JOURNAL
卷 31, 期 10, 页码 2296-2308出版社
WILEY
DOI: 10.1038/emboj.2012.105
关键词
Mmi1; RITS; RNAi; sexual differentiation; S. pombe
资金
- French Ministry of Research
- l'Association pour la Recherche contre le Cancer (ARC)
- Japan Society for the Promotion of Science
- international Human Frontier Science Programme Organization (HFSPO)
- ARC
- Institut National de la Sante Et de la Recherche Medicale (INSERM)
- European Research Council (ERC)
- Grants-in-Aid for Scientific Research [21227007] Funding Source: KAKEN
RNA interference (RNAi) silences gene expression by acting both at the transcriptional and post-transcriptional levels in a broad range of eukaryotes. In the fission yeast Schizosaccharomyces pombe the RNA-Induced Transcriptional Silencing (RITS) RNAi complex mediates heterochromatin formation at non-coding and repetitive DNA. However, the targeting and role of RITS at other genomic regions, including protein-coding genes, remain unknown. Here we show that RITS localizes to specific meiotic genes and mRNAs. Remarkably, RITS is guided to these meiotic targets by the RNA-binding protein Mmi1 and its associated RNA surveillance machinery that together degrade selective meiotic mRNAs during vegetative growth. Upon sexual differentiation, RITS localization to the meiotic genes and mRNAs is lost. Large-scale identification of Mmi1 RNA targets reveals that RITS subunit Chp1 associates with the vast majority of them. In addition, loss of RNAi affects the effective repression of sexual differentiation mediated by the Mmi1 RNA surveillance machinery. These findings uncover a new mechanism for recruiting RNAi to specific meiotic genes and suggest that RNAi participates in the control of sexual differentiation in fission yeast. The EMBO Journal (2012) 31, 2296-2308. doi: 10.1038/emboj.2012.105; Published online 20 April 2012
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