期刊
EMBO JOURNAL
卷 31, 期 1, 页码 214-227出版社
WILEY
DOI: 10.1038/emboj.2011.361
关键词
cytoskeleton; membrane trafficking; molecular motors; plant pathogen
资金
- BBSRC [BB/G00465X/1]
- DFG Graduate School [1216]
- BBSRC [BB/H019774/1, BB/G00465X/1] Funding Source: UKRI
- MRC [MC_U117570592] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H019774/1, BB/G00465X/1] Funding Source: researchfish
- Medical Research Council [1106254, MC_U117570592] Funding Source: researchfish
Plant infection by pathogenic fungi requires polarized secretion of enzymes, but little is known about the delivery pathways. Here, we investigate the secretion of cell wall-forming chitin synthases (CHSs) in the corn pathogen Ustilago maydis. We show that peripheral filamentous actin (F-actin) and central microtubules (MTs) form independent tracks for CHSs delivery and both cooperate in cell morphogenesis. The enzyme Mcs1, a CHS that contains a myosin-17 motor domain, is travelling along both MTs and F-actin. This transport is independent of kinesin-3, but mediated by kinesin-1 and myosin-5. Arriving vesicles pause beneath the plasma membrane, but only similar to 15% of them get exocytosed and the majority is returned to the cell centre by the motor dynein. Successful exocytosis at the cell tip and, to a lesser extent at the lateral parts of the cell requires the motor domain of Mcs1, which captures and tethers the vesicles prior to secretion. Consistently, Mcs1-bound vesicles transiently bind F-actin but show no motility in vitro. Thus, kinesin-1, myosin-5 and dynein mediate bi-directional motility, whereas myosin-17 introduces a symmetry break that allows polarized secretion. The EMBO Journal (2012) 31, 214-227. doi: 10.1038/emboj.2011.361; Published online 25 October 2011
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