期刊
EMBO JOURNAL
卷 30, 期 12, 页码 2420-2430出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/emboj.2011.148
关键词
methylation; ribosome; rRNA; snoRNA; snoRNP
资金
- BBSRC
- Wellcome Trust
- EMBO long-term fellowship
- Marie Curie EIF fellowship
- BBSRC [BB/F006853/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F006853/1] Funding Source: researchfish
2'-O-methylation of eukaryotic ribosomal RNA (r)RNA, essential for ribosome function, is catalysed by box C/D small nucleolar (sno)RNPs. The RNA components of these complexes (snoRNAs) contain one or two guide sequences, which, through base-pairing, select the rRNA modification site. Adjacent to the guide sequences are protein-binding sites (the C/D or C'/D' motifs). Analysis of >2000 yeast box C/D snoRNAs identified additional conserved sequences in many snoRNAs that are complementary to regions adjacent to the rRNA methylation site. This 'extra base-pairing' was also found in many human box C/D snoRNAs and can stimulate methylation by up to five-fold. Sequence analysis, combined with RNA-protein crosslinking in Saccharomyces cerevisiae, identified highly divergent box C'/D' motifs that are bound by snoRNP proteins. In vivo rRNA methylation assays showed these to be active. Our data suggest roles for non-catalytic subunits (Nop56 and Nop58) in rRNA binding and support an asymmetric model for box C/D snoRNP organization. The study provides novel insights into the extent of the snoRNA-rRNA interactions required for efficient methylation and the structural organization of the snoRNPs. The EMBO Journal (2011) 30, 2420-2430. doi: 10.1038/emboj.2011.148; Published online 10 May 2011
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