期刊
EMBO JOURNAL
卷 30, 期 4, 页码 744-755出版社
WILEY
DOI: 10.1038/emboj.2010.344
关键词
Arabidopsis; meiosis; meiotic recombination; retinoblastoma
资金
- Temasek Life Sciences Laboratory
- Singapore Millenium Foundation
- Biological and Biotechnology Research Council, UK
- Biotechnology and Biological Sciences Research Council [BB/E006469/1] Funding Source: researchfish
- BBSRC [BB/E006469/1] Funding Source: UKRI
We have analysed the role of RBR (retinoblastoma related), the Arabidopsis homologue of the tumour suppressor Retinoblastoma protein (pRb), during meiosis. We characterise the rbr-2 mutation, which causes a loss of RBR in male meiocytes. The rbr-2 plants exhibit strongly reduced fertility, while vegetative growth is generally unaffected. The reduced fertility is due to a meiotic defect that results in reduced chiasma formation and subsequent errors in chromosome disjunction. Immunolocalisation studies in wild-type meiocytes reveal that RBR is recruited as foci to the chromosomes during early prophase I in a DNA double-strand-break-dependent manner. In the absence of RBR, expression of several meiotic genes is reduced. The localisation of the recombinases AtRAD51 and AtDMC1 is normal. However, localisation of the MutS homologue AtMSH4 is compromised. Additionally, polymerisation of the synaptonemal complex protein AtZYP1 is abnormal. Together, these data indicate that loss of RBR during meiosis results in a reduction of crossover formation and an associated failure in chromosome synapsis. Our results indicate that RBR has an important role in meiosis affecting different aspects of this complex process. The EMBO Journal (2011) 30, 744-755. doi:10.1038/emboj.2010.344; Published online 7 January 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据